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KMID : 0378019740170030345
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New Medical Journal
1974 Volume.17 No. 3 p.345 ~ p.351
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Influence of some toxic agents on Paneth Cells of Mice
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Abstract
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This experiment was performed in order to study morphological responses of Paneth cells to some systemic toxic agents.
One hundred and five healthy adult male mice(D. D. O. /Lee, white mice) weighing about 20g each were divided into two groups-normal and toxic. Toxic agents used in this experiment were mercuric chloride (24 mg/kg, intravenously), uranyl nitrate(40 mg/kg, intravenously), potassium bichromate (30 mg/kg, intravenously), sodium potassium tartrate (400 mg/kg, subcutaneously) and carbon tetrachloride (4 ml/kg, subcutaneously), and a single dose of each of them was injected to the animals. The mercuric chloride-treated animals were, sacrificed on the 12 th, 18 th, .24 th and 48th hour after the injection and the mice treated with the other agents were sacrificed on the I st, 2 nd, 3 rd and 5 th day after the injection.
Duodenal specimens of all the experimental animals were fixed in 10% neutral formalin solution, embedded in paraffin wax, sectioned at a thickness of 5 p, and stained with hematoxylin-eosin, Masson´s trichrome or Best´s carmine. Morphological changes of Paneth cells were observed, and the average numbers of the cells were counted in the longitudinally well-sectioned twenty intestinal glands and semiquantitative granulation indices were obtained arithmatically weighted method to 3 cell types classified according to the degree of granularity of the cells.
The obtained results were as follows:
1. Numbers of Paneth cells did not show any significant changes following administration of systemic toxic agents but granular indices showed distinct decrease in the early stage, on the 1st day in the case of mercuric chloride, uranyl nitrate and sodium potassium tartrate, and on the 2 nd day in the case of potassium bichromate and carbon tetrachloride, and then slowly recovered to normal state at the end of the experiment.
2. After the injection of toxic agents, Paneth cells showed irregularities in shape, decrease of granules, irregular size and dispersed distribution of granules, weakness of stain reactions, and increase of vacuoles.
3. Considering the above findings, systemic toxic agents depressed activity of Paneth cells in the early stage after the administration and recovery of granularities of the cells seemed to be slow.
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